Fluorescence quantitative PCR technology involves adding specific probes labeled with fluorescent dyes or fluorescent markers to the PCR reaction system. By continuously accumulating fluorescent signals, real-time monitoring of the entire PCR amplification reaction process is achieved. Based on the changes in reaction time and fluorescence signals, a curve is drawn, and corresponding software is used to analyze the target substance, calculate the initial concentration of the target substance in the test sample, and apply the results for analysis. Fluorescence quantitative PCR technology has been widely applied in various fields such as molecular biology, human and animal medicine, food testing, and environmental monitoring.